egfp 2 Search Results


90
Dawley Inc sd-tg(rosa-egfp)2-4reh rats
Sd Tg(Rosa Egfp)2 4reh Rats, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sd-tg(rosa-egfp)2-4reh rats/product/Dawley Inc
Average 90 stars, based on 1 article reviews
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90
GenScript corporation egfp-2
Egfp 2, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/egfp-2/product/GenScript corporation
Average 90 stars, based on 1 article reviews
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Ribobio co pexp rb mam egfp2 h gja1
miR‐454‐3p targeted <t>GJA1</t> (Cx43). (A) Venn diagram was used to achieve the potential genes that miR‐454‐3p may target the binding region. (B) Schematic illustration of the predicted binding sites between GJA1 and miR‐454‐3p, and mutation of potential miR‐454‐3p binding sequence in GJA1. (C) Dual luciferase assay was applied to assess the seed‐matching sites or mutant sites between GJA1 and miR‐454‐3p in HEK‐293T. (D, E) The protein levels of Cx43 in U87MG and A172 cells transfected with miR‐454‐3p mimics, miR‐NC mimics or miR‐454‐3p inhibitor, miR‐NC inhibitor. GAPDH was the control. Relative density values were counted using image J software. (F) The mRNA expression level of GJA1 transfected with miR‐NC mimics or miR‐454‐3p mimics. (G) Relative expression of GJA1 in normal brain and GBM tissues, normalized to GAPDH. (H) The Pearson's correlation analysis was performed to analyze the correlation between GJA1 and miR‐454‐3p. * p < 0.05; ** p < 0.01; *** p < 0.01.
Pexp Rb Mam Egfp2 H Gja1, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pexp rb mam egfp2 h gja1/product/Ribobio co
Average 90 stars, based on 1 article reviews
pexp rb mam egfp2 h gja1 - by Bioz Stars, 2026-03
90/100 stars
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90
Oxford Nanopore aav6.2-cmv-egfp (2.0 kb)
miR‐454‐3p targeted <t>GJA1</t> (Cx43). (A) Venn diagram was used to achieve the potential genes that miR‐454‐3p may target the binding region. (B) Schematic illustration of the predicted binding sites between GJA1 and miR‐454‐3p, and mutation of potential miR‐454‐3p binding sequence in GJA1. (C) Dual luciferase assay was applied to assess the seed‐matching sites or mutant sites between GJA1 and miR‐454‐3p in HEK‐293T. (D, E) The protein levels of Cx43 in U87MG and A172 cells transfected with miR‐454‐3p mimics, miR‐NC mimics or miR‐454‐3p inhibitor, miR‐NC inhibitor. GAPDH was the control. Relative density values were counted using image J software. (F) The mRNA expression level of GJA1 transfected with miR‐NC mimics or miR‐454‐3p mimics. (G) Relative expression of GJA1 in normal brain and GBM tissues, normalized to GAPDH. (H) The Pearson's correlation analysis was performed to analyze the correlation between GJA1 and miR‐454‐3p. * p < 0.05; ** p < 0.01; *** p < 0.01.
Aav6.2 Cmv Egfp (2.0 Kb), supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/aav6.2-cmv-egfp (2.0 kb)/product/Oxford Nanopore
Average 90 stars, based on 1 article reviews
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Image Search Results


miR‐454‐3p targeted GJA1 (Cx43). (A) Venn diagram was used to achieve the potential genes that miR‐454‐3p may target the binding region. (B) Schematic illustration of the predicted binding sites between GJA1 and miR‐454‐3p, and mutation of potential miR‐454‐3p binding sequence in GJA1. (C) Dual luciferase assay was applied to assess the seed‐matching sites or mutant sites between GJA1 and miR‐454‐3p in HEK‐293T. (D, E) The protein levels of Cx43 in U87MG and A172 cells transfected with miR‐454‐3p mimics, miR‐NC mimics or miR‐454‐3p inhibitor, miR‐NC inhibitor. GAPDH was the control. Relative density values were counted using image J software. (F) The mRNA expression level of GJA1 transfected with miR‐NC mimics or miR‐454‐3p mimics. (G) Relative expression of GJA1 in normal brain and GBM tissues, normalized to GAPDH. (H) The Pearson's correlation analysis was performed to analyze the correlation between GJA1 and miR‐454‐3p. * p < 0.05; ** p < 0.01; *** p < 0.01.

Journal: Cancer Reports

Article Title: Inhibiting lncRNA NEAT1 Increases Glioblastoma Response to TMZ by Reducing Connexin 43 Expression

doi: 10.1002/cnr2.70031

Figure Lengend Snippet: miR‐454‐3p targeted GJA1 (Cx43). (A) Venn diagram was used to achieve the potential genes that miR‐454‐3p may target the binding region. (B) Schematic illustration of the predicted binding sites between GJA1 and miR‐454‐3p, and mutation of potential miR‐454‐3p binding sequence in GJA1. (C) Dual luciferase assay was applied to assess the seed‐matching sites or mutant sites between GJA1 and miR‐454‐3p in HEK‐293T. (D, E) The protein levels of Cx43 in U87MG and A172 cells transfected with miR‐454‐3p mimics, miR‐NC mimics or miR‐454‐3p inhibitor, miR‐NC inhibitor. GAPDH was the control. Relative density values were counted using image J software. (F) The mRNA expression level of GJA1 transfected with miR‐NC mimics or miR‐454‐3p mimics. (G) Relative expression of GJA1 in normal brain and GBM tissues, normalized to GAPDH. (H) The Pearson's correlation analysis was performed to analyze the correlation between GJA1 and miR‐454‐3p. * p < 0.05; ** p < 0.01; *** p < 0.01.

Article Snippet: GenePharma in China provided lncRNA NEAT1 siRNA‐1, ‐2, ‐3, and control siRNA (si‐NC), whereas miR‐NC mimics, miR‐454‐3p mimics, miR‐454‐3p inhibitor, and miR‐NC inhibitor, pEXP‐RB‐Mam‐EGFP2‐h‐GJA1, pEXP‐RB‐Mam‐EGFP2‐h‐control, GJA1 siRNA, and NC siRNA were acquired from RIBOBIO located in China.

Techniques: Binding Assay, Mutagenesis, Sequencing, Luciferase, Transfection, Control, Software, Expressing

Cx43 is responsible for NEAT1‐mediated TMZ resistance. (A) The Pearson's correlation analysis was performed to analyze the correlation between NEAT1 and GJA1. (B) The mRNA expression level of GJA1 in U87MG cells with shNC and shNEAT1 group, ** p < 0.01. (C, F) The protein levels of Cx43, BCL2, and BAX in U87MG cells with shNC + Vector, shNEAT1 + Vector or shNEAT1 + Cx43. *** p < 0.001, compared to shNC + Vector group; ### p < 0.001, compared to shNEAT1 + Vector group. (D) The mRNA expression level of NEAT1 transfected with GJA1 siRNA or NC siRNA. *** p < 0.01. (E) Cell proliferation assays of shNC and shNEAT1 in U87MG and A172 cells transfected with Cx43 plasmid or Vector, with TMZ treatment. *** p < 0.001, compared to shNC + Vector group; ## p < 0.01, ### p < 0.001, compared to shNEAT1 + Vector group. (G, H) Flow cytometric analysis of si‐NC and si‐NEAT1 in U87MG and A172 cells transfected with Cx43 plasmid or vector, with TMZ treatment (100 μM, 48 h). ** p < 0.01, *** p < 0.001. (I) The SLDT assay was conducted to detect the influence of shNC + Vector, shNEAT1 + Vector or shNEAT1 + Cx43 on gap junction intercellular communication, which was indicated by the dye spreading area. Scale bar = 100 μm.

Journal: Cancer Reports

Article Title: Inhibiting lncRNA NEAT1 Increases Glioblastoma Response to TMZ by Reducing Connexin 43 Expression

doi: 10.1002/cnr2.70031

Figure Lengend Snippet: Cx43 is responsible for NEAT1‐mediated TMZ resistance. (A) The Pearson's correlation analysis was performed to analyze the correlation between NEAT1 and GJA1. (B) The mRNA expression level of GJA1 in U87MG cells with shNC and shNEAT1 group, ** p < 0.01. (C, F) The protein levels of Cx43, BCL2, and BAX in U87MG cells with shNC + Vector, shNEAT1 + Vector or shNEAT1 + Cx43. *** p < 0.001, compared to shNC + Vector group; ### p < 0.001, compared to shNEAT1 + Vector group. (D) The mRNA expression level of NEAT1 transfected with GJA1 siRNA or NC siRNA. *** p < 0.01. (E) Cell proliferation assays of shNC and shNEAT1 in U87MG and A172 cells transfected with Cx43 plasmid or Vector, with TMZ treatment. *** p < 0.001, compared to shNC + Vector group; ## p < 0.01, ### p < 0.001, compared to shNEAT1 + Vector group. (G, H) Flow cytometric analysis of si‐NC and si‐NEAT1 in U87MG and A172 cells transfected with Cx43 plasmid or vector, with TMZ treatment (100 μM, 48 h). ** p < 0.01, *** p < 0.001. (I) The SLDT assay was conducted to detect the influence of shNC + Vector, shNEAT1 + Vector or shNEAT1 + Cx43 on gap junction intercellular communication, which was indicated by the dye spreading area. Scale bar = 100 μm.

Article Snippet: GenePharma in China provided lncRNA NEAT1 siRNA‐1, ‐2, ‐3, and control siRNA (si‐NC), whereas miR‐NC mimics, miR‐454‐3p mimics, miR‐454‐3p inhibitor, and miR‐NC inhibitor, pEXP‐RB‐Mam‐EGFP2‐h‐GJA1, pEXP‐RB‐Mam‐EGFP2‐h‐control, GJA1 siRNA, and NC siRNA were acquired from RIBOBIO located in China.

Techniques: Expressing, Plasmid Preparation, Transfection

Journal: Cancer Reports

Article Title: Inhibiting lncRNA NEAT1 Increases Glioblastoma Response to TMZ by Reducing Connexin 43 Expression

doi: 10.1002/cnr2.70031

Figure Lengend Snippet:

Article Snippet: GenePharma in China provided lncRNA NEAT1 siRNA‐1, ‐2, ‐3, and control siRNA (si‐NC), whereas miR‐NC mimics, miR‐454‐3p mimics, miR‐454‐3p inhibitor, and miR‐NC inhibitor, pEXP‐RB‐Mam‐EGFP2‐h‐GJA1, pEXP‐RB‐Mam‐EGFP2‐h‐control, GJA1 siRNA, and NC siRNA were acquired from RIBOBIO located in China.

Techniques: Sequencing

Journal: Cancer Reports

Article Title: Inhibiting lncRNA NEAT1 Increases Glioblastoma Response to TMZ by Reducing Connexin 43 Expression

doi: 10.1002/cnr2.70031

Figure Lengend Snippet:

Article Snippet: GenePharma in China provided lncRNA NEAT1 siRNA‐1, ‐2, ‐3, and control siRNA (si‐NC), whereas miR‐NC mimics, miR‐454‐3p mimics, miR‐454‐3p inhibitor, and miR‐NC inhibitor, pEXP‐RB‐Mam‐EGFP2‐h‐GJA1, pEXP‐RB‐Mam‐EGFP2‐h‐control, GJA1 siRNA, and NC siRNA were acquired from RIBOBIO located in China.

Techniques: